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Friday, November 6, 2020 | History

2 edition of characterization of some bacillus megaterium phages. found in the catalog.

characterization of some bacillus megaterium phages.

Russell Siboo

characterization of some bacillus megaterium phages.

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  • 31 Currently reading

Published in [Toronto] .
Written in English

    Subjects:
  • Bacillus megatherium,
  • Bacteriophages.

  • Edition Notes

    ContributionsToronto, Ont. University.
    Classifications
    LC ClassificationsLE3 T525 MSA 1962 S53
    The Physical Object
    Pagination92 leaves.
    Number of Pages92
    ID Numbers
    Open LibraryOL14746863M

      Bacillus anthracis, the etiological agent of anthrax, is a gram‐positive, spore‐forming rod, with colonies exhibiting a unique ground‐glass appearance, and lacking hemolysis and addition to these phenotypes, several others traits are characteristic of B. anthracis such as susceptibility to gamma phage, the presence of two virulence plasmids (pX01 and pX02), and specific cell.   The Bacillus megaterium, Escherichia coli, Klebsiella pneumonia and Bacillus marinus (AUX36, AUX53, AUX and AUX respectively) were selected by salkowski reagent as auxin producers. Colorimetric analysis showed that all selected isolates produced maximum auxin in the presence of tryptophan at 37 C and pH 7 after 72 hours of incubation. This chapter focuses on spore germination principally as it is understood in Bacillus subtilis. The protease that initiates small acid-soluble spote proteins (SASP) hydrolysis during spore germination is discussed. While SASP degradation may be slower than cortex degradation, the latter event is not necessary for SASP hydrolysis. Spore outgrowth begins after spore germination, but in a spore.


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characterization of some bacillus megaterium phages. by Russell Siboo Download PDF EPUB FB2

A new Bacillus megaterium bacteriophage is characterized. It is a tailed phage with regular polyhedral head belonging to Bradley's group B. Head and tail dimensions are and nm, respectively. Lysis was restricted to strains of B. megaterium. No antigenic relationship with pumilus phage FP-1 or subtilis phage FS-1 was observed.

The phage is sensitive to 60°C and moderately Cited by: 6. Based on physiological traits and genetic sequencing, three bacterial isolates were identified as Bacillus megaterium (strain NHO-1), Bacillus pumilus (strain NHO-2), and Paenibacillus polymyxa (strain NHO-3).

These bacteria grew in synthetic media of low pH (pH ) while some survived ethanol concentrations up to 15% v/ by: 3. These phages, designated MP9 to MP50, were tested for host-range on several strains of B.

megaterium and 13 other Bacillus species. All the phages only infected B. megaterium. The φBM phage was shown to be able to infect Bacillus megaterium and two other Bacillus species and has no effect on other tested bacteria.

φBM was stable over the pH range ofchloroform. Several Bacillus bacteriophages have been isolated, but very few Bacillus megaterium bacteriophages have been characterized. In this study, we describe the biological characteristics, whole genome sequences, and their annotations for two new isolates of the B.

megaterium bacteriophages (BM5 and BM10), which were isolated from Egyptian soil by: 2. Introduction. Bacillus megaterium (Firmicutes) is ubiquitous in nature.

It is recognized as an endophyte and a potential biocontrol agent for plant diseases (Kildea et al., ).Furthermore, it is known to produce penicillin, amidase, various baking amylases and is used for the biotechnological production of pyruvate, vitamin B12, and so on (Bunk et al., ).

Phage isolates BMC1, BMC2, BMC5, BMC6, BMC7 and BMC8 (population 1) were found to be infectious to B. megaterium among the four species of s, phages BMC3, BMC4, BMC9 and BMC10 (Population 2) were found to be infectious to B. megaterium and B. subtilis. Moreover, B.

circulans and B. polymexa were resistant to all phage isolates under study, whereas, B. megaterium. Bacillus megaterium has been industrially employed for more than 50 years, as it possesses some very useful and unusual enzymes and a high capacity for the production of exoenzymes. It is also a desirable cloning host for the production of intact proteins, as it does not possess external alkaline proteases and can stably maintain a variety of plasmid vectors.

Zbl. Bakt. Abt, (), [InstituteofMicrobiology,Federal UniversityofRiodeJ aneiro, Riode Janeiro, Brasil] Isolation and Characterization of Bacillus pumilus Phages from Brazilian Soil J.

VAN ELSAS and ELISA G. Biochemical characterization of the isolates revealed the presence of Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Bacillus polymyxa, Bacillus coagulans, Lactobacillus plantarum, Staphylococccus aureus, Aspergillus niger, A.

parasiticus, Rhizopus stolonifer, Rhodotorula spp. and Saccharomyces cerevisiae as microorganisms. In this research, isolation, screening, and characterization of six isolates of K solubilizing bacteria (KSB) from some Iranian soils were carried out.

The ability of all isolates were tested in three treatments including acid-leached soil, biotite, and muscovite by analyzing the soluble K content after 5 days of incubation at 28 ± 2°C. Characterization of B.

megaterium FDU A strain of B. megaterium tolerant to arid condition (15% PEG (w/w), aw ) was isolated from plaque areas on the surface of a leaflet in an old book, and was named as FDU Abstract.

Romig, W. (University of California, Los Angeles), and A. ion and preliminary characterization of bacteriophages for Bacillus subtilis.J. Bacteriol. – —A characterization of some bacillus megaterium phages. book procedure for direct isolation of phages for Bacillus subtilis from soil was developed.

Phage enrichment was accomplished by growing streptomycin-resistant B. subtilis in medium. The Genetic Map of Bacillus megaterium, p In Sonenshein A, Hoch J, Losick R (ed), Bacillus subtilis and Other Gram-Positive Bacteria. ASM Press, Washington, DC.

doi: /ch   The genus Bacillus consisted of Bacillus cereus (%, with 7 phenotypes), Bacillus megaterium (%, with 3 phenotypes), Bacillus subtilus (%, with 6 phenotypes) and Bacillus sp. (%, with 1 phenotype). The genus Micrococcusre grouped 08 phenotypes. The relative abundance of isolates demonstrated that in all leaf litters and soils.

Some strains of Bacillus cereus, Bacillus megaterium and Bacillus sphaericus are capable of biotransformations of inexpensive natural steroidal substrates into high‐value therapeutic compounds (Manosroi et al., ; Wadhwa and Smith, ).

JOURNAL OF INVERTEBRATE PATHOLOGY() Some Properties of a Bacteriophage from Bacillus larvae T. GoCHNAUER Entomology Research Institute, Canada Department o f Agriculture, Ottawa, Canadal Received J Cultures of Bacillus larvae isolated from a wide variety of honey-bee sources in the northern plains area were uniformly sensitive to.

GENOME ANNOUNCEMENT. Bacteriophage Eldridge is a member of the Myoviridae family, isolated on Bacillus megaterium as the host organism.B.

megaterium is a Gram-positive, rod-shaped, endospore-forming bacterium.B. megaterium is a nonpathogenic host used for the biotechnological production of substances, including vitamin B 12, amylases, and penicillin amylase ().

Bacillus cereus is a spore-forming, Gram-positive bacterium and is a major food-borne pathogen. A B. cereus-specific bacteriophage PBC4 was isolated from the soil of a stock farm, and its genome was 4 belongs to the Siphoviridae family and has a genome consisting of 80 bp-long double-stranded DNA, including genes and two tRNAs.

LysPBC4, the endolysin of PBC4, has an. A Bacillus subtilis phage, SP3, and its bacterial host were examined by high resolution electron microscopy. The structural components of negatively s.

A previously described soil-isolated Bacillus subtilis bacteriophage, GS 1, was further is a tailed phage with regular head morphology and a discrete base plate.

GS 1 belongs to morphological group A1 of Bacillus phages. Its tail measured nm and the head width was 92 nm. Endolysins are phage-encoded enzymes implicated in the breaching of the bacterial cell wall at the end of the viral cycle. This study focuses on the endolysins of Deep-Blue (PlyB) and Deep-Purple (PlyP32), two phages preying on the Bacillus cereus group.

Both enzymes exhibit a typical modular organization with an enzymatically active domain (EAD) located in the N-terminal and a cell wall.

Bacillus cereus is a pathogen related with diarrhoeal or emetic food poisoning cases, of which the latter caused by the cereulide-producing isolates are more severe with several reported lethal cases.

It is therefore necessary to develop an effective strategy to prevent the propagation of B. cereus in the food supply.

In this study, three autolysins from the cereulide-producing B. cereus group. Bacillus species including Bacillus cereus, Bacillus subtilis, Bacillus anthracoides, and Bacillus megaterium were refractory to infection.

Conclusions Data reported here provide further insight into the shared genomic architecture, host range specificity, and molecular evolution of these rare B. cereus group phages. TAKAHASHI I. Genetic transduction in Bacillus subtilis.

Biochem Biophys Res Commun. Jun 28; – TAKAHASHI I. Transducing phages for Bacillus subtilis. J Gen Microbiol. May; – Vary JC. Spore germination of Bacillus megaterium QM B mutants. J Bacteriol. Oct; (1)– [PMC free article]. Ten phages of Bacillus polymyxa were isolated from four different Brazilian soils.

All were dsDNA-containing phages belonging to Bradley types A and B. Data obtained from electron microscopy and tests of resistance against physical and chemical agents showed that the isolates could be distributed among six different groups.

Host range data were in agreement with this classification. Purchase Genetics and Biotechnology of Bacilli, Volume 2 - 1st Edition. Print Book & E-Book.

ISBNThe first generalized transducing bacteriophage reported for Bacillus megaterium has been characterized. Optimum conditions for lysate production and transduction procedures were established so. Abstract. The polydisperse circular deoxyribonucleic acid (DNA) molecules which comprise up to 30% of the total extractable DNA of Bacillus megaterium strain have been purified and partially characterized.

Banding in cesium chlorideethidium bromide by “gradient relaxation” in a fixed-angle rotor provided good resolution of circular and chromosomal DNAs for preparative separations.

Tikhonenko AS, Belyaeva NN, Ivánovics G. Electron microscopy of phages liberated by megacin A producing lysogenic Bacillus megaterium strains.

Acta Microbiol Acad Sci Hung. ; 22 (1)– Von Tersch MA, Carlton BC. Bacteriocin from Bacillus megaterium ATCC comparative studies with megacin A J Bacteriol. Abstract.

Brodetsky, Anna M. (University of California, Los Angeles), and W. terization of Bacillus subtilis bacteriophages. Bacteriol. – —A group of six phages, SP5, SP6, SP7, SP8, SP9, and SP13, which use the Marburg strain of Bacillus subtilis as host was characterized.

These phages, referred to as group 1, were examined for the following properties. Nine new species of tailed Bacillus phages, based on morphological and physicochemical properties, are defined. Stokrová J, Forstová J, Meyer J () Correlation of physical maps and some genetic functions in the genomes of the κ-ϑ phage family ofBacillus licheniformis () Characterization of a newBacillus megaterium.

Host-range and partial characterization of several new bacteriophages for Bacillus megaterium QM b Vary PS, Halsey WF. J Gen Virol, 51(pt 1), 01 Nov Cited by: 8 articles | PMID: We review some aspects of the rapid isolation of, screening for and characterization of jumbo phages, i.e., phages that have dsDNA genomes longer than Kb.

The first aspect is that, as plaque-supporting gels become more concentrated, jumbo phage plaques become smaller. Dilute agarose gels are better than conventional agar gels for supporting plaques of both jumbo phages and, prospectively.

Some Bacillus species were described after16 further genera have been established to accommodate species previously assigned to Bacillus, and. Buy Characterization of marine pigmented Bacillus pumilus SF on FREE SHIPPING on qualified orders Characterization of marine pigmented Bacillus pumilus SF Saad Fakhry, Ezio Ricca, Ahmed Jessim: : Books.

Whole genome nucleotide and amino acid comparison of the Bacillusfamily of phages reveals 12 diverse clusters and 14 singletons.

In order to determine the relationship of the 93 extant, fully-sequenced Bacillus phages as of June 1, we analyzed the published phage genomes by methods similar to those of Hatfull et al.

[15, 16], including whole genome dot plot analysis, pairwise average. Host range specificity study showed that GV1 was lytic on thirteen thermophilic Bacillus-like strains tested, including strains of Geobacillus stearothermophilus, G. thermoglucosidasius, B. licheniformis, Anoxybacillus idirlerensis, and A.

kuwalawohkensis. However, GV1 failed to infect a mesophilic strain of Bacillus megaterium. ABSTRACT. A salt‐tolerant acid protease was purified from the culture broth of Bacillus megaterium KLP‐98 with a molecular weight of 64 kDa.

K m and k cat values of the protease were mg/mL and s −1 against azocasein as a substrate, respectively. The optimal temperature for protease activity was 60C but optimal stability temperature was below 40C.

Custom B5 Contains Bacillus megaterium. Here’s a brief introduction to two key beneficial plant bacteria, Bacillus subtilis and Bacillus megaterium. Bacillus subtilis. Bacillus subtilis, or B. sub, is a key beneficial plant bacteria.B. subtilis plays a role in replenishing soil nutrients through both the carbon cycle and the nitrogen cycle.

To aid the carbon cycle B. subtilis degrades thatch. Molecular characterization of plasmid pBM from Bacillus megaterium QM B Appld Envrion. Microbiol. 71(6) Rosso, M.-L. and P.S. Vary. Distribution of Bacillus megaterium QM B plasmids among other Bacillus megaterium strains and Bacillus species.

Plasmid. Fu, Guifen, Patricia S. Vary, and Chhiu-Tsu Lin. -Unsure what % agar to make the semisolid. % seems too runny, % could prevent the bacteria and phage from mixing.

Each batch seems to come out differently as well.This chapter reviews current information on the nature and regulation of the genes involved in the synthesis of riboflavin, biotin, folic acid, thiamine, lipoic acid, and pantothenic acid in Bacillus subtilis, and of cobalamin in Bacillus megaterium.

More is known about riboflavin (rib) genes in B. subtilis than in any other microorganism. It is possible that flavin mononucleotide (FMN) itself.